The Rockefeller University » Laboratory of Bacterial Pathogenesis and Immunology

Surface Proteins on Gram-Positive Bacteria

C-terminal-Linked Surface Proteins from Gram-Positive Bacteria
	NAME / GENE	FUNCTION / NAME	ORGANISM	LPXTG	REFERENCE	ACCESS#
1.	emm6	M protein	S. pyogenes	LPSTG	(24)	M11338
2.	emm5	M protein	S. pyogenes	LPSTG	(40)	M20374
3.	emm12	M protein	S. pyogenes	LPSTG	(52)	U02342
4.	emm24	M protein	S. pyogenes	LPSTG	(41)	M19031
5.	emm49	M protein	S. pyogenes	LPSTG	(19)	M23689
6.	emm57	M protein	S. pyogenes	LPSTG	(37)	X60959
7.	emm2	M protein	S. pyogenes	LPSTG	(3)	X61276
8.	emm3	M protein	S. pyogenes	LPSTG	(31)	Z21845
9.	ARP2	IgA binding protein	S. pyogenes	LPSTG	(3)	X61276
10.	ARP4	IgA binding protein	S. pyogenes	LPSTG	(14)	X15198
11.	Mrp4	IgG/fibrinogen binding	S. pyogenes	LPSTG	(44)	M87831
12.	FcRA	Fc binding protein	S. pyogenes	LPSTG	(20)	M22532
13.	Prot H	Human IgG Fc binding	S. pyogenes	LPSTG	(17)	M29398
14.	SCP	C5a peptidase	S. pyogenes	LPTTN	(7)	J05229
15.	T6	Protease resistant protein	S. pyogenes	LPSTG	(54)	M32978
16.	sof22	Serum opacity factor	S. pyogenes	LPASG	(50)	U02290
17.	Sfb	Fibronectin binding	S. pyogenes	LPATG	(61)	X67947
18.	ZAG	Binds ?2 M, Alb, IgG	S. zooepidemicus	LPTTG	(27)	U02290
19.	PrtF	Fibronectin binding	S. pyogenes	LPATG	(55)	L10919
20.	PAM	Plasmin binding	S. pyogenes	LPSTG	(1)	Z22219
21.	Prot L	Light chain binding	P. magnus	LPKAG	(30)	M86697
22.	PAB	HSA binding	P. magnus	LPEAG	(11)	X77864
23.	bac	IgA binding protein	Gr. B strep	LPYTG	(21)	X58470
24.	bca	alpha C antigen	Gr. B strep	LPATG	(39)	M97256
25.	fnbA	Fibronectin binding	S. dysgalactiae	LPQTG	(36)	Z22150
26.	fnbB	Fibronectin binding	S. dysgalactiae	LPAAG	(36)	Z22151
27.	Fnz	Fibronectin binding	S. equi	LPQTS	(42)	Y17116
28.	SeM	M-like	S. equi	LPSTG	(62)	U73162
29.	SzPSe	M-like	S. equi	LPQTS	(62)	U73163
30.	Prot G	IgG binding protein	Gr. G strep	LPTTG	(46)	X06173
31.	EmmG1	M protein	Gr. G Strep	LPSTG	(9)	M95774
32.	DG12	Albumin binding protein	Gr. G strep	LPSTG	(57)	M95520
33.	GfbA	Fibronectin binding	Gr. G. strep	LPATG	(33)	U31115
34.	MRP	Surface protein*	S. suis	LPNTG	(58)	X64450
35.	PAc	Surface protein	S. mutans	LPNTG	(45)	X14490
36.	spaP	Surface protein	S. mutans	LPNTG	(32)	X17390
37.	spaA	Surface protein	S. sobrinus	LPATG	(63)	D90354
38.	wapA	Wall-associated protein A	S. mutans	LPSTG	(13)	M19347
39.	fruA	Fructosidase	S. mutans	LPDTG	(5)	L03358
40.	Sec10	Surface protein	E. fecalis	LPQTG	(29)	M64978
41.	Asc10	Surface protein	E. fecalis	LPKTG	(29)	M64978
42.	asa1	Aggregation substance	E. fecalis	LPQTG	(16)	X17214
43.	Prot A	IgG binding protein	S. aureus	LPETG	(18)	X00342
44.	FnBPb	Fibronectin binding	S. aureus	LPETG	(56)	J04151
45.	FnBPa	Fibronectin binding	S. aureus	LPETG	(28)	X62992
46.	cna	Collagen binding protein	S. aureus	LPKTG	(49)	M81736
47.	clfA	Clumping factor	S. aureus	LPDTG	(38)	Z18852
48.	EDIN	Epidermal cell inhibitor	S. aureus	LPRGT	(25)	NA**
49.	prtM	Cell wall protease	L. paracasei	LPKTA	(23)	M83946
50.	wg2	Cell wall protease	S. cremoris	LPKTG	(34)	M24767
51.	inlA	Internalization protein	L. monocytogenes	LPTTG	(15)	M67471
52.	Fimbriae	Type 1 fimbriae	A. viscosis	LPLTG	(67)	M32067
53.	Fimbriae	Type 2 fimbriae	A. naeslundii	LPLTG	(66)	M21976
54.	FimA	Type 2 fimbriae	A. naeslundii	LPLTG	(68)	AF019629
55.	Hyal1	Hyaluronidase	S. pneumoniae	LPQTG	(2)	L20670
56.	nanA	Neuraminidase	S. pneumoniae	LPETG	(6)	X72967
57.	glnA	Glutamine synthetase	Group B strep	LPATL	(59)	U61271
58.	Protein F2	Fibronectin binding	S. pyogenes	LPATG	(26)	U31980
59.	Fbe	Fibrinogen binding	S. epidermidis	LPDTG	(43)	Y17116
60.	InlC2	Internalin-like	L. monocytogenes	LPTAG	(12)	U77368
61.	InlD	Internalin-like	L. monocytogenes	LPTAG	(12)	U77368
62.	InlE	Internalin-like	L. monocytogenes	LP I TG	(12)	U77368
63.	InlF	Internalin-like	L. monocytogenes	LPKTG	(12)	U77367
64.	SfBP1	Fibronectin binding	S. pyogenes	LPXTG	(53)	AF071083
65.	dex	Dextranase	S. sobrinus	LPKTG	(64)	M96978
66.	pad1	Pheromone	E. fecalis	LPHTG	(65)	X62658
67.	FAI	Fibrinogen/albumin/IgG	Group C streptococcus	LPSTG	(60)	NA
68.	SfbII	Fibronectin binding	S. pyogenes	LPASG	(35)	X83303
69.	spy0130	Minor pilin protein	S. pyogenes	LPSTG	(70)
70.	spy0128	Major pilin protein	S. pyogenes	EVPTG	(70)

Surface proteins attached by other mechanisms:
1.	ftf	Fructosyl transferase	S. salivarius	(51)
2.	pspA	Surface protein	S. pneumoniae	(69)
3.	aas	Adhesin/autolytic	S. saprophiticus	(22)
4.	FbpA	Fibrinogen binding	S. aureus	(8)
5.	FBP54	Fibronectin/fibrinogen bind	S. pyogenes	(10)
6.	Fib	Fibronectin binding	S. aureus	(4)
7.	SEN	Surface enolase	S. pyogenes	(48)
8.	SDH	Surface dehydrogenase	S. pyogenes	(47)
* Surface proteins - proteins that have been identified to have a C-terminal anchor motif but the function is unknown.
** NA - Not available

Reference List
1.	Berge, A. and U. Sjobring. 1993. PAM, a novel plasminogen-binding protein from Streptococcus pyogenenes. J.Biol.Chem. 268(34):25417-25424.
2.	Berry, A.M., R.A. Lock, S.M. Thomas, D.P. Rajan, D. Hansman, and J.C. Paton. 1994. Cloning and nucleotide sequence of the Streptococcus pneumoniae hyaluronidase gene and purification of the enzyme from recombinant Escherichia coli. Infect.Immun. 62(3):1101-1108.
3.	Bessen, D.E. and V.A. Fischetti. 1992. Nucleotide sequences of two adjacent M and M-like protein genes of group A streptococci: different RNA transcript levels and identification of a unique IgA-binding protein. Infect.Immun.60:124-135.
4.	Boden, M.K. and J.-I. Flock. 1994. Cloning and characterization of a gene for a 19kDa fibrinogen-binding protein from staphylococcus aureus. Molecular Microbiology 12:599-606.
5.	Burne, R.A. and J.E.C. Penders. 1993. Characterization of the Streptococcus mutans GS-5 fruA gene encoding Exo-beta-D-fructosidase. Infect.Immun. 60(11):4621-4632.
6.	Camara, M., G.J. Boulnois, P.W. Andrew, and T.J. Mitchell. 1994. A neuraminidase from Streptococcus pneumoniae has the features of a surface protein. Infect.Immun. 62(9):3688-3695.
7.	Chen, C.C. and P.P. Cleary. 1990. Complete nucleotide sequence of the streptococcal C5a peptidase gene of Streptococcus pyogenes. J.Biol.Chem. 265:3161-3167.
8.	Cheung, A.I., S.J. Projan, R.E. Edelstein, and V.A. Fischetti. 1995. Cloning, expression, and nucleotide sequence of a staphylococcus aureus (fbpA)encoding a fibrinogen-binding protein. Infection and Immunity63:1914-1920.
9.	Collins, C.M., A. Kimura, and A.L. Bisno. 1992. Group G streptococcal M protein exhibits structural features analogous to those of class I M protein of group A streptococci. Infect.Immun. 60:3689-3696.
10.	Courtney, H.S., Y. Li, J.B. Dale, and D.L. Hasty. 1994. Cloning, sequencing, and expression of a fibronectin/fibrinogen-binding protein from group A streptococci. Infect.Immun. 62(9):3937-3946.
11.	de Chateau, M. and L. Bjorck. 1994. Protein PAB, a mosaic albumin-binding bacterial protein representing the first contemporary example of module shuffling. J.Biol.Chem. 269(16):12147-12151.
12.	Dramsi, S., P. Dehoux, M. Lebrun, P.L. Goossens, and P. Cossart. 1998. Identification of four new members of the internalin multigene family of Listeria monocytogenes EGD. Infection and Immunity 65:1615-1625.
13.	Ferretti, J.J., R.R.B. Russell, and M.L. Dao. 1989. Sequence analysis of the wall-associated protein precursor of Streptococcus mutans antigen A. Molec.Microbiol. 3:469-478.
14.	Frithz, E., L.-O. Heden, and G. Lindahl. 1989. Extensive sequence homology between IgA receptor and M protein in Streptococcus pyogenes. Molec.Microbiol. 3:1111-1119.
15.	Gaillard, J.L., P. Berche, C. Frehel, E. Gouin, and P. Cossart. 1991. Entry of L. monocytogenes into Cells Is Mediated by a Repeat Protein Analgous to Surface Antigens from Gram-Positive, Extracellular Pathogens. Cell 65:1127-1141.
16.	Galli, D., F. Lottspeich, and R. Wirth. 1990. Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1. Molec.Microbiol. 4:895-904.
17.	Gomi, H., T. Hozumi, S. Hattori, C. Tagawa, F. Kishimoto, and L. Bjorck. 1990. The gene sequence and some properties of protein H. J.Immunol. 144:4046-4052.
18.	Guss, B., M. Uhlen, B. Nilsson, M. Lindberg, J. Sjoquist, and J. Sjodahl. 1984. Region X, the cell-wall-attachment part of staphylococcal protein A. Eur.J.Biochem. 138:413-420.
19.	Haanes, E.J. and P.P. Cleary. 1989. Identification of a divergent M protein gene and an M protein related gene family in serotype 49 Streptococcus pyogenes. J.Bacteriol. 171:6397-6408.
20.	Heath, D.G. and P.P. Cleary. 1989. Fc-receptor and M protein genes of group A streptococci are products of gene duplication. Proc.Natl.Acad.Sci.U.S.A. 86:4741-4745.
21.	Heden, L.-O., E. Frithz, and G. Lindahl. 1991. Molecular characterization of an IgA receptor from Group B Streptococci: sequence of the gene, identification of a proline-rich region with unique structure and isolation of N-terminal fragments with IgA-binding capacity. Eur.J.Immunol. 21:1481-1490.
22.	Hell, W., H.-G.W. Meyer, and S.G. Gatermann. 1998. Cloning of aas, a gene encoding a staphylococcus saprophyticus surface protein with adhesive and autolytic properties. Molec.Microbiol. 29:871-881.
23.	Holck, A. and H. Naes. 1992. Cloning, sequencing and expression of the gene encoding the cell-envelope-associated proteinase from Lactobaccillus paracasei subsp. paracasei NCDO 151. J.Gen.Microbiol. 138:1353-1364.
24.	Hollingshead, S.K., V.A. Fischetti, and J.R. Scott. 1986. Complete nucleotide sequence of type 6 M protein of the group A streptococcus: repetitive structure and membrane anchor. J.Biol.Chem. 261:1677-1686.
25.	Inoue, S., M. Sugai, Y. Murooka, S.-Y. Paik, Y.-M. Hong, H. Ohgai, and H. Suginaka. 1991. Molecular cloning and sequencing of the epidermal cell differentiation inhibitor gene from Staphylococcus aureus. Biochem.Biophys.Res.Commun. 174:459-464.
26.	Jaffe, J., S. Natanson-Yaron, M.G. Caparon, and E. Hanski. 1996. Protein F2, a novel fibronectin-binding protein from Streptococcus pyogenes, possesses two binding domains. Molec.Microbiol. 21:373-384.
27.	Johnsson, H., H. Lindmark, and B. Guss. 1995. A protein G-related cell surface protein in Streptococcus zooepidemicus. Infect.Immun. 63:2968-2975.
28.	Jonsson, K., C. Signas, H.-P. Muller, and M. Lindberg. 1991. Two different genes encode fibronectin binding proteins in Staphylococcus aureus. Eur.J.Biochem. 202:1041-1048.
29.	Kao, S.-M., S.B. Olmsted, A.S. Viksnins, J.C. Gallo, and G.M. Dunny. 1991. Molecular and genetic analysis of a region of plasmid pCF10 containing positive control genes and structural genes encoding surface proteins involved in pheromone-inducible conjugation in Enterococcus faecalis. J.Bacteriol. 173:7650-7664.
30.	Kastern, W., U. Sjobring, and L. Bjorck. 1992. Structure of peptostreptococcal protein L and identification of a repeated immunoglobulin light chain-binding domain. J.Biol.Chem. 267 (18):12820-12825.
31.	Katsukawa, C. 1994. Cloning and nucleotide sequence of type 3 M protein gene (emm3) consisting of an N-terminal variable portion and C-terminal conserved C repeat regions: relation to other genes of Streptococcus pyogenes. Journal of Japanese Association for Infectious Diseases 68:698-705.
32.	Kelly, C., P. Evans, L. Bergmeier, S.F. Lee, F.A. Progulske, A.C. Harris, A. Aitken, A.S. Bleiweis, and T. Lerner. 1990. Sequence analysis of a cloned streptococcal surface antigen I/II. FEBS Lett. 258:127-132.
33.	Kline, J. B., Xu, S., Bisno, A. L., and Collins, C. M. Identification of a fibronectin-binding protein (GfbA) in pathogenic group G streptococci. Infect.Immun. 64(6), 2122-2129. 1996.
34.	Kok, J., K.J. Leenhouts, A.J. Haandrikman, A.M. Ledeboer, and G. Venema. 1988. Nucleotide sequence of the cell wall proteinase gene of Streptococcus cremoris Wg2. Appl.Environ.Microbiol. 54:231-238.
35.	Kreikemeyer, B., S.R. Talay, and G.S. Chhatwal. 1995. Characterization of a novel fibronectin-binding surface protein in group A streptococci. Molec.Microbiol. 17:137-145.
36.	Lindgren, P.-E., M.J. McGavin, C. Signas, B. Guss, S. Gurusiddappa, M. Hook, and M. Lindberg. 1993. Two different genes coding for fibronectin-binding proteins from Streptococcus dysgalactiae. The complete nucleotide sequences and characterization of the binding domain. Eur.J.Biochem. 214:819-827.
37.	Manjula, B.N., K.M. Khandke, T. Fairwell, W.A. Relf, and K.S. Sripakash. 1991. Heptad motifs within the distal subdomain of the coiled-coil rod region of M protein from rheumatic fever and nephritis associated serotypes of group A streptococci are distinct from each other: nucleotide sequence of the M57 gene and relation of the deduced amino acid sequence of other M proteins. J.Protein Chem. 10:369-383.
38.	McDevitt, D., P. Francois, P. Vaudaux, and T.J. Foster. 1994. Molecular charcterization of the clumping factor (fibrinogen receptor) of Staphylococcus aureus. Molecular Microbiology 11(12):237-248.
39.	Michel, J.L., L.C. Madoff, K. Olson, D.E. Kling, D.L. Kasper, and F.M. Ausubel. 1992. Large, identical, tandem repeating units in the C protein alpha antigen gene, bca, of group B streptococci. Proc.Natl.Acad.Sci.U.S.A. 89:10060-10064.
40.	Miller, L., L. Gray, E.H. Beachey, and M.A. Kehoe. 1988. Antigenic variation among group A streptococcal M proteins: Nucleotide sequence of the serotype 5 M protein gene and its relationship with genes encoding types 6 and 24 M proteins. J.Biol.Chem. 263:5668-5673.
41.	Mouw, A.R., E.H. Beachey, and V. Burdett. 1988. Molecular evolution of streptococcal M protein: Cloning and nucleotide sequence of type 24 M protein gene and relation to other genes of Streptococcus pyogenes. J.Bacteriol. 170:676-684.
42.	Nilsson, A., L. Frykberg, J.-I. Flock, L. Pei, M. Lindberg, and B. Guss. 1998. A fibrinogen-binding protein of Staphylococcus epidermis. Infection and Immunity 66:2666-2673.
43.	Nilsson, M., L. Frykberg, J.I. Flock, L. Pei, M. Lindberg, and B. Guss. 1998. A fibrinogen-binding protein of Staphylococcus epidermidis. Infect.Immun. 66:2666-2673.
44.	O'Toole, P., L. Stenberg, M. Rissler, and G. Lindahl. 1992. Two major classes in the M protein family in group A streptococci. Proc.Natl.Acad.Sci.U.S.A. 89:8661-8665.
45.	Okahashi, N., C. Sasakawa, S. Yoshikawa, S. Hamada, and T. Koga. 1989. Molecular characterization of a surface protein antigen gene from serotype c Streptococcus mutans implicated in dental caries. Molec.Microbiol. 3:673-678.
46.	Olsson, A., M. Eliasson, B. Guss, B. Nilsson, U. Hellman, M. Lindberg, and M. Uhlen. 1987. Structure and evolution of the repetitive gene encoding streptococcal protein G. Eur.J.Biochem. 168:319-324.
47.	Pancholi, V. and V.A. Fischetti. 1993. Glyceraldehyde-3-phosphate dehydrogenase on the surface of group A streptococci is also an ADP-ribosylating enzyme. Proc.Natl.Acad.Sci.U.S.A. 90 :8154-8158.
48.	Pancholi, V. and V.A. Fischetti. 1998. a-enolase, a novel strong plasmin(ogen) binding protein of the surface of pathogenic streptococci. J.Biol.Chem. 273:14503-14515.
49.	Patti, J.M., H. Jonsson, B. Guss, L.M. Switalski, K. Wiberg, M. Lindberg, and M. Hook. 1992. Molecular characterizations and expression of a gene encoding a Staphylococcus aureus collagen adhesin. J.Biol.Chem. 267:4766-4772.
50.	Rakonjac, J.V., J.C. Robbins, and V.A. Fischetti. 1995. DNA sequence of the serum opacity factor of group A streptococci: Identification of a fibronectin-binding repeat domain. Infect.Immun. 63:622-631.
51.	Rathsam, C., P.M. Giffard, and N.A. Jacques. 1993. The cell-bound fructosyltransferase of Streptococcus salivarius: the carboxyl terminus specifies attachment in a Streptococcus gordonii model system. J.Bacteriol. 175:4520-4527.
52.	Robbins, J.C., J.G. Spanier, S.J. Jones, W.J. Simpson, and P.P. Cleary. 1987. Streptococcus pyogenes type 12 M protein regulation by upstream sequences. J.Bacteriol. 169:5633-5640.
53.	Rocha, C and Fischetti, V. A. 1999. Isolation and characterization of a fibronectin binding protein on the surface of group A streptococci. Infect. Immun. (Press)
54.	Schneewind, O., K.F. Jones, and V.A. Fischetti. 1990. Sequence and structural characterization of the trypsin-resistant T6 surface protein of group A streptococci. J.Bacteriol. 172:3310-3317.
55.	Sela, S., A. Aviv, A. Tovi, I. Burstein, M.G. Caparon, and E. Hanski. 1993. Protein F: an adhesin of Streptococcus pyogenes binds fibronectin via two distinct domains. Molec.Microbiol. 10(5):1049-1055.
56.	Signas, C., G. Raucci, K. Jonsson, P. Lindgren, G.M. Anantharamaiah, M. Hook, and M. Lindberg. 1989. Nucleotide sequence of the gene for fibronectin-binding protein from Staphylococcus aureus: Use of this peptide sequence in the synthesis of biologically active peptides. Proc.Natl.Acad.Sci.U.S.A. 86:699-703.
57.	Sjobring, U. 1992. Isolation and molecular characterization of a novel albumin-binding protein from group G streptococci. Infect.Immun. 60:3601-3608.
58.	Smith, H.E., U. Vecht, A.L.J. Gielkens, and M.A. Smits. 1992. Cloning and nucleotide sequence of the gene encoding the 136-kilodalton surface protein (muramidase-released protein) of Streptococcus suis type 2. Infect.Immun. 60:2361-2367.
59.	Suvorov, A.N., A.E. Flores, and P. Ferrieri. 1997. Cloning of the glutamine synthetase gene from group B streptococci. Infect.Immun. 65:191-196.
60.	Talay, S.R., M.P. Grammel, and G.S. Chhatwal. 1996. Structure of a group C streptoccal protein that binds to fibrinogen, albumin and immunoglobulin G via overlapping modules. Biochem.J. 315:577-582.
61.	Talay, S.R., P. Valentin-Weigand, P.G. Jerlstrom, K.N. Timmis, and G.S. Chhatwal. 1992. Fibronectin-binding protein of Streptococcus pyogenes: sequence of the binding domain involved in adherence of streptococci to epithelial cells. Infect.Immun. 60:3837-3844.
62.	Timoney, J.F., S.C. Artiushin, and J.S. Boschwitz. 1997. Comparison of the sequences and functions of Streptococcus equi M-like proteins SeM and SzPSe. Infect.Immun. 65:3600-3605.
63.	Tokuda, M., N. Okahashi, I. Takahashi, M. Nakai, S. Nagaoka, M. Kawagoe, and T. Koga. 1991. Complete nucleotide sequence of the gene for a surface protein antigen of Streptococcus sobrinus. Infect.Immun. 59:3309-3312.
64.	Wanda, S.-Y. and R. Curtiss III. 1994. Purification and characterization of Streptococcus sobrinus dextranase produced in recombinant Escherichia coli and sequence analysis of the dextranase gene. J.Bacteriol. 176(13):3839-3850.
65.	Weidlich, G., R. Wirth, and D. Galli. 1992. Sex pheromone plasmid pADI-encoded surface exclusion protein of Enterococcus faecalis . Mol.Gen.Genet. 233:161-168.
66.	Yeung, M.K. and J.O. Cisar. 1988. Cloning and nucleotide sequence of a gene for Actinomyces naeslundii 45 type 2 fimbriae. J.Bacteriol. 170:3803-3809.
67.	Yeung, M.K. and J.O. Cisar. 1990. Sequence homology between the subunits of two immunologically and functionally distinct types of fimbriae of Actinomyces spp. J.Bacteriol. 172:2462-2468.
68.	Yeung, M.K., J.A. Donkersloot, J.O. Cisar, and P.A. Ragsdale. 1998. Identification of a gene involved in assembly of Actinomyces naeslundii T14V type 2 fimbriae. Infection and Immunity 66:1482-1491.
69.	Yother, J. and D.E. Briles. 1992. Structural properties and evolutionary relationships of PspA, a surface protein of Streptococcus pneumoniae, as revealed by sequence analysis. J.Bacteriol. 174:601-609.
70.	Scott, J.R. and D. Zähner. 2006. Pili with strong attachments: Gram-positive bacteria do it differently. Molec Microbiol 62:320–330.

Back